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Collaboration: Isthmus, Valorization and Applications Laboratory (Genoscope)
About 3500 thiamine enzymes (transketolases, acetolactate synthases, decarboxylases, etc.) have been cloned from 200 bacterial genomes and one metagenome. As a first step, these enzymes have been expressed and screened for their potential decarboxylase activity on a panel of over twenty keto-acids. Over one hundred candidates which could decarboxylate at least one of these substrates, even marginally, have been detected, and are in the process of being characterized individually.